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KMID : 0380020090240060508
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Korean Journal of Biotechnology and Bioengineering
2009 Volume.24 No. 6 p.508 ~ p.514
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Isolation and Characterization of Catalase-producing Bacteria from Soil
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Han Kyung-Ah
Lee Jong-Il
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Abstract
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This study investigated the production of catalase from Bul-kyo soil bacteria through fermentation process. Isolation and selection of bacteria was performed through chemical and physiological analysis. Catalases were produced from bacteria which belong to 3 different species (Bacillaceae bacterium BKBChE-1, Bacillus sp. BKBChE-2, Bacillus flexus BKBChE-3) confirmed by using 16S rDNA sequence method. The catalases were found to be stable in the temperature range of 30℃-60℃ for BKBChE-1, BKBChE-2 and BKBChE-3 and also in the pH range of 9.0-12.0 for BKBChE-1 and BKBChE-3. Long-term stability of the catalases was about 20 days at 4℃. However, BKBChE-2 has kept its activity over 30 days at 4℃.
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KEYWORD
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Catalase, Hydrogen peroxide, Bacillus sp., Identification, 16S rDNA
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